Schistosome and Snail Recipes

iBge Medium pH 7.2

Filter sterilize (0.22 um filter) and store at 4C

To prepare cBge medium, add 10 mL of heat inactivated FBS and 1 mL of 100X pen/strep to 89 mL iBge.

Note: iBge is very easily contaminated

Note: Do not freeze cBge Medium

Note: Bge cells are sensitive to the quality of FBS. Several lots of FBS may have to be screened. It has been known that investigators using Bge cell lines have soon lost it due to the unsuitability of their FBS.

Hansen's Buffered Saline pH 7.5-7.7

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Chernin's Balanced Salt Solution (CBSS)

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Filter sterilize (0.22 um filter) and store at 4C
To make CBSS+, add 1 g each of glucose and trehalose

Trypsin Solution 0.05%

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Filter sterilize (0.22 um filter).